Functional and Mechanistic Study of Dot1l in Mouse Embryonic Hematopoiesis

Early mammalian erythropoiesis requires the Dot1L methyltransferase.

Histone methylation is an important regulator of gene expression; its coordinated activity is critical in complex developmental processes such as hematopoiesis. Disruptor of telomere silencing 1-like (DOT1L) is a unique histone methyltransferase that specifically methylates histone H3 at lysine 79. We analyzed Dot1L-mutant mice to determine influence of this enzyme on embryonic hematopoiesis. M...

HEMATOPOIESIS AND STEM CELLS Early mammalian erythropoiesis requires the Dot1L methyltransferase

Histone methylation is an important regulator of gene expression; its coordinated activity is critical in complex developmental processes such as hematopoiesis. Disruptor of telomere silencing 1-like (DOT1L) is a unique histone methyltransferase that specifically methylates histone H3 at lysine 79. We analyzed Dot1L-mutant mice to determine influence of this enzyme on embryonic hematopoiesis. M...

DOT1L promotes angiogenesis through cooperative regulation of VEGFR2 with ETS-1

Histone methyltransferase DOT1L is implicated in various biological processes including cell proliferation, differentiation and embryogenesis. Gene ablation of Dot1l results in embryonic lethality and cardiovascular defects including decreased vasculature. However, how DOT1L might contribute to the development of vasculature is not clear. Here, we report that DOT1L is required for angiogenesis....

Functional Concentrations of BMP4 on Differentiation of Mouse Embryonic Stem Cells to Primordial Germ Cells

When a gold standard is made of tin.

Given the complete lack of H3K79 methyl-ation it is perhaps surprising that the defect in the Dot1L-deficient embryos is rather specific and mainly appears to affect angiogenesis and hematopoiesis, although Dot1L is expressed in many other tissues and cell types. 6 However , Dot1L function may be important for the development and function of other organs and cell types at later stages during de...